Document 3195
 DOCN  M94A3195
 TI    Mycobacterium avium infection and murine AIDS.
 DT    9412
 AU    Perronne C; Grassi F; Levacher M; Cohen Y; Maslo C; Chau F; Sinet M;
       Vilde JL; Pocidalo JJ; Service des Maladies Infectieuses, Hopital
       Bichat-Claude; Bernard, Paris, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):131 (abstract no. PA0142). Unique
       Identifier : AIDSLINE ICA10/94369380
 AB    OBJECTIVE: To study in mice the interaction between a retroviral
       infection (murine AIDS) and Mycobacterium avium complex (MAC) infection.
       METHODS: The murine leukemia virus LP-BM5 was inoculated to C57BL/6 mice
       by i.v. injection either 8 weeks before an i.v. challenge with 10(3) or
       10(6) MAC (strain MO-1) (coinfection 1), or 10 days after an i.v.
       challenge with 10(3) MAC (coinfection 2). During coinfection 2, the
       phenotypic alterations in blood lymphocyte subsets were monitored by
       FACS-analysis using the combination of anti-CD4, CD8 and Ly5 with
       anti-Ly6 monoclonal antibodies. RESULTS: During coinfection 1, LP-BM5
       infection tended to decrease the mycobacterial growth, the difference
       reaching statistical significance for the lower inoculum (10(3) MAC) (p
       < 0.001). During coinfection 2, LP-BM5 did not modify the course of MAC
       infection. Compared to uninfected mice, LP-BM5 induced a strong
       activation of CD4, CD8 and B lymphocytes (Ly5+) (p < 0.001), as
       evidenced by the up-regulation of Ly6 expression, while MAC did not. The
       coinfection provided an immunologic pattern similar to that of LP-BM5
       infection alone. DISCUSSION & CONCLUSIONS: Immune dysfunctions caused by
       LP-BM5 infection did not exacerbate but rather decreased MAC infection.
       Lymphocyte activation may account for this interaction. In our
       experience, this model of retroviral infection of mice does not seem to
       be a reliable model of immunosuppression for the study of MAC infection.
 DE    Animal  Antibodies, Monoclonal  Antigens, CD4/BLOOD  Antigens, CD8/BLOOD
       Antigens, Ly/BLOOD  B-Lymphocyte Subsets/IMMUNOLOGY  Flow Cytometry
       Lymphocyte Subsets/*IMMUNOLOGY  Mice  Mice, Inbred C57BL  Murine
       Acquired Immunodeficiency Syndrome/*COMPLICATIONS/  IMMUNOLOGY
       *Mycobacterium avium  Tuberculosis/*COMPLICATIONS/IMMUNOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).